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Van Holde-Weischet Method

Sedimentation is a transport process propotional to the first power of time, while diffusion is a transport process which is proportional to the square-root power of time. At infinity1, transport due to diffusion will be neglible compared to transport due to Sedimentatin.

Applications

The van Holde-Weischet Analysis is applicable for the following examples:

  • Concentration dependend non-ideality of the sedimenatin coefficient

  • Aggregation and Irreversible self-Association

  • Composition Analysis

  • Reversibly self-associating systems vs. non-interacting systems

  • Stoichiometry of association

  • Relative quantification of individual components

  • Conformational information

Van Holde-Weischet with UltraScan LIMS iII

  1. van Holde, K. E. and W. O. Weischet. (1978). Boundary Analysis of Sedimentation Velocity Experiments with Monodisperse and Paucidisperse Solutes. Biopolymers, 17:1387-1403 ↩